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This membrane is then incubated in a solution which contains a DNA probe, which is complementary to the target DNA.

Based on their sizes, smaller molecules will migrate further down the agarose gel sheet and on the contrary, the bigger ones will remain on the upper part of the gel.Īfter the separation is complete, the DNA on the gel is transferred onto a nylon membrane. This type of gel is particularly used for nucleic acid separation because of its reticulation ability, which means that the gel is able to easily separate bigger and smaller molecules.īecause they are charged negatively, the nucleic acids will respond to the electric field created by the electrophoresis. These fragments are then placed on the top of an agarose gel. The protocol begins with the extraction of DNA from cells, which then leads to the production of DNA fragments. It is a procedure which has been developed to identify particular sequences of DNA. Southern Blotting was first discovered and set up by Edward M. In this article we will get to know the goals and steps of each technique.

Three main techniques are used: Southern, Northern and Western Blot.Īll three of them are based on similar protocols, but the outcome varies, as well as some of the steps of the protocol.

In the field of molecular biology, different blotting techniques are used to detect, identify and quantify the presence of a specific protein or to identify nucleic acid sequences.īlotting refers to the process during which macromolecules are transferred from a gel onto the solid surface of a membrane, which will in other words, aspirate the information from the surface of the gel.